DPTE Program

The Dutch Program for Tissue Engineering and the Microarray Facility Nijmegen

The Microarray Facility Nijmegen will be the core facility for microarray-based expression profiling within the DPTE. For this purpose an Affymetrix Genechip system has been acquired together with personnel for performing the expression profiling experiments (technician, ing. Simon van Reijmersdal) and for data analysis (bioinformatician, dr. Michael Egmont-Petersen). Additional equipment (including an Agilent Bioanalyzer and a Nanodrop system for RNA and labelling QC) is available within the facility, as well as commercial (including Genespring from Silicon Genetics) and in-house developed software for data analysis. The work will be supervised by prof. dr. Ad Geurts van Kessel and dr. Joris A. Veltman.

The ultimate goal of this facility within the DPTE is to allow high quality quantitative expression profiling data from postnatal stem cells. Therefore, we will optimize expression profiling protocols for postnatal stem cell applications and establish a MIAME compliant data management system for automatic acquisition and storage of primary expression profiling data, obtained both from microarray and quantitative PCR experiments. In addition, we will set up an expert system for high throughput data mining.

Participants of the DPTE who are planning to include microarray-based expression profiling experiments in their project are urged to contact dr. Joris Veltman ( mail , phone 024 3614941) at the earliest stage possible, in order to implicate the Microarray Facility Nijmegen in their study design and to discuss the (im)possibilities, and to allow an efficient planning of the experiments to be performed.

For further information about the DPTE, see www.dpte.nl.